Ontology highlight
ABSTRACT:
SUBMITTER: Lange OF
PROVIDER: S-EPMC3390869 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Lange Oliver F OF Rossi Paolo P Sgourakis Nikolaos G NG Song Yifan Y Lee Hsiau-Wei HW Aramini James M JM Ertekin Asli A Xiao Rong R Acton Thomas B TB Montelione Gaetano T GT Baker David D
Proceedings of the National Academy of Sciences of the United States of America 20120625 27
We have developed an approach for determining NMR structures of proteins over 20 kDa that utilizes sparse distance restraints obtained using transverse relaxation optimized spectroscopy experiments on perdeuterated samples to guide RASREC Rosetta NMR structure calculations. The method was tested on 11 proteins ranging from 15 to 40 kDa, seven of which were previously unsolved. The RASREC Rosetta models were in good agreement with models obtained using traditional NMR methods with larger restrain ...[more]