Model of the trimeric fiber and its interactions with the pentameric penton base of human adenovirus by cryo-electron microscopy.

ABSTRACT: Adenovirus invades host cells by first binding to host receptors through a trimeric fiber, which contains three domains: a receptor-binding knob domain, a long flexible shaft domain, and a penton base-attachment tail domain. Although the structure of the knob domain associated with a portion of the shaft has been solved by X-ray crystallography, the in situ structure of the fiber in the virion is not known; thus, it remains a mystery how the trimeric fiber attaches to its underlying pentameric penton base. By high-resolution cryo-electron microscopy, we have determined the structure of the human adenovirus type 5 (Ad5) to 3.6-Å resolution and have reported the full atomic models for its capsid proteins, but not for the fiber whose density cannot be directly interpreted due to symmetry mism

SUBMITTER: Liu H 

PROVIDER: S-EPMC3392045 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature