Binding of regulatory subunits of cyclic AMP-dependent protein kinase to cyclic CMP agarose.

ABSTRACT: The bacterial adenylyl cyclase toxins CyaA from Bordetella pertussis and edema factor from Bacillus anthracis as well as soluble guanylyl cyclase ?(1)?(1) synthesize the cyclic pyrimidine nucleotide cCMP. These data raise the question to which effector proteins cCMP binds. Recently, we reported that cCMP activates the regulatory subunits RI? and RII? of cAMP-dependent protein kinase. In this study, we used two cCMP agarose matrices as novel tools in combination with immunoblotting and mass spectrometry to identify cCMP-binding proteins. In agreement with our functional data, RI? and RII? were identified as cCMP-binding proteins. These data corroborate the notion that cAMP-dependent protein kinase may serve as a cCMP target.

SUBMITTER: Hammerschmidt A 

PROVIDER: S-EPMC3392273 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature