Ontology highlight
ABSTRACT:
SUBMITTER: Goers Sweeney E
PROVIDER: S-EPMC3392440 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Structure (London, England : 1993) 20120614 7
pH sensing is crucial for survival of most organisms, yet the molecular basis of such sensing is poorly understood. Here, we present an atomic resolution structure of the periplasmic portion of the acid-sensing chemoreceptor, TlpB, from the gastric pathogen Helicobacter pylori. The structure reveals a universal signaling fold, a PAS domain, with a molecule of urea bound with high affinity. Through biophysical, biochemical, and in vivo mutagenesis studies, we show that urea and the urea-binding s ...[more]