Shp2 activates Fyn and Ras to regulate RBL-2H3 mast cell activation following Fc?RI aggregation.

ABSTRACT: The protein-tyrosine phosphatase (PTP) Shp2 has been implicated in many immunoreceptor signaling pathways, but its role in immunoreceptor Fc?RI signaling, which leads to the activation of mast cells and blood basophils, is still largely undefined. Using Shp2 knockdown RBL-2H3 (RBL) mast cells, we here reported that Shp2 is required for the activation of RBL cells induced by Fc?RI. Fc?R?-evoked degranulation, calcium mobilization, and synthesis of cytokine transcripts (IL-1?, IL-10, and monocyte chemoattractant protein 1 (MCP-1)) were reduced in Shp2 knockdown RBL cells. Signaling regulatory mechanism investigation using immunoblotting, immunoprecipitation, and GST pull-down assay reveals that the down-regulation of Shp2 expression in RBL cells leads to decreased activities of Fyn, PLC?, JNK, p38MAPK, and Ras/Erk1/2 after Fc?R? aggregation. Further studies suggest that Paxillin phosphoryaltion was also impaired, but PAG phosphorylation was normal after Fc?R? stimulation as a consequence of the inhibition of Shp2 expression in RBL cells. Collectively, our data strongly indicate that Shp2 is essential for the activation of RBL cells in response to Fc?R? aggregation. Shp2 regulates this process through Fyn and Ras with no involvement of PAG. In addition, we identify Paxillin as an indirect substrate of Shp2 in Fc?R?-initiated signaling of RBL cells.

SUBMITTER: Fang X 

PROVIDER: S-EPMC3393662 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature