Ontology highlight
ABSTRACT:
SUBMITTER: Alves AP
PROVIDER: S-EPMC3395163 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Alves Analiza P AP Lorenzen Marcé D MD Beeman Richard W RW Foster John E JE Siegfried Blair D BD
Journal of insect science (Online) 20100101
To test the efficacy of RNA interference (RNAi) as a method for target-site screening in Diabrotica virgifera virgifera LeConte (Coleptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model, Tribolium castaneum. Here the cloning of the D. v. vergifera orthologs of laccase 2 (DvvLac2) and chitin synthase 2 (DvvCHS2) is reported. Injection of DvvLac2-specific double-stranded RNA resulted in prevention of post-molt c ...[more]