Microbiome complexity and Staphylococcus aureus in chronic rhinosinusitis.

ABSTRACT:

Objectives/hypothesis

The aim of this study was to compare microbiological culture-based and culture-independent (16S rRNA gene sequencing) methodologies for pathogen identification in chronic rhinosinusitis (CRS) patients. We hypothesized that bacterial culture and DNA sequencing would yield largely concurrent results, although sequencing would detect greater bacterial diversity, and the sinonasal microbiomes of CRS patients would differ in composition and diversity compared with non-CRS controls.

Study design

Cross-sectional observational study.

Methods

Middle meatus swabs from CRS patients collected during endoscopic sinus surgery were analyzed by both clinical culture and broad-range analysis of 16S rRNA gene pyrosequences.

Results

A total of 21 swab sample

SUBMITTER: Feazel LM 

PROVIDER: S-EPMC3398802 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature