Imaging the distribution of sodium dodecyl sulfate in skin by confocal Raman and infrared microspectroscopy.

ABSTRACT:

Purpose

To image SDS distribution across different skin regions, to compare the permeability difference between porcine and human skin, and to evaluate the interaction between SDS and skin.

Methods

Full thickness porcine and human skin was treated with acyl chain perdeuterated SDS (SDS-d(25)) at room temperature and at 34 °C for 3, 24 and 40 h. SDS distribution in skin was monitored by confocal Raman and IR microspectroscopic imaging. Permeation profiles of SDS-d(25) in skin were derived from the band intensities of the CD(2) stretching vibrations. The interaction between SDS and skin was monitored through the CH(2) and CD(2) stretching frequencies and the Amide I and II spectral region.

Results

SDS-d(25) penetrates both porcine and human skin in a time and temperatur

SUBMITTER: Mao G 

PROVIDER: S-EPMC3399083 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature