Ontology highlight
ABSTRACT:
SUBMITTER: Zhang C
PROVIDER: S-EPMC3400429 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Nature biotechnology 20110601 7
Mammalian RNA complexity is regulated through interactions of RNA-binding proteins (RBPs) with their target transcripts. High-throughput sequencing together with UV-crosslinking and immunoprecipitation (HITS-CLIP) is able to globally map RBP-binding footprint regions at a resolution of ~30-60 nucleotides. Here we describe a systematic way to analyze HITS-CLIP data to identify exact crosslink sites, and thereby determine protein-RNA interactions at single-nucleotide resolution. We found that reve ...[more]