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Stem cell-related markers in primary breast cancers and associated metastatic lesions.


ABSTRACT: It has been reported previously that: (1) normal-breast epithelial cells that are CD24-/44+ express higher levels of stem/progenitor cell-associated genes; (2) cancer cells that have undergone epithelial to mesenchymal transition display CD24-/44+ cell-surface expression, a marker for breast cancer stem cells; (3) loss of E-cadherin is a preliminary step in epithelial to mesenchymal transition; and (4) vimentin is a marker of mesenchymal phenotype. We hypothesized that stem cell subpopulations would be more frequent in metastatic than in primary tumors. Therefore we assessed by immunohistochemical analysis, tissue microarrays containing tissue from primary and associated metastatic breast cancers for expression of CD24, CD44, E-cadherin and vimentin to evaluate candidate cancer-initiating cell populations in breast cancer subtypes and metastatic lesions. The occurrence of CD24-/44+ and CD24+/44- cells did not differ in primary vs matched lymph node or distant and locoregional metastatic lesions; E-cadherin expression was decreased in primary vs lymph node metastases (P=0.018) but not decreased in distant and locoregional metastases relative to primary tumor, whereas vimentin, was more frequently expressed in lymph node and distant and locoregional metastases (P=0.013, P=0.004) than in matched primary cancers. Thus, the frequency of CD24-/44+ cells does not differ in metastases relative to the primary breast cancer but differs by tumor stage and subtype.

SUBMITTER: Guler G 

PROVIDER: S-EPMC3400504 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Stem cell-related markers in primary breast cancers and associated metastatic lesions.

Guler Gulnur G   Balci Serdar S   Costinean Stefan S   Ussakli Cigdem Himmetoglu CH   Irkkan Cigdem C   Suren Dinc D   Sari Ebru E   Altundag Kadri K   Ozisik Yavuz Y   Jones Susie S   Bacher Jason J   Shapiro Charles L CL   Huebner Kay K  

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 20120302 7


It has been reported previously that: (1) normal-breast epithelial cells that are CD24-/44+ express higher levels of stem/progenitor cell-associated genes; (2) cancer cells that have undergone epithelial to mesenchymal transition display CD24-/44+ cell-surface expression, a marker for breast cancer stem cells; (3) loss of E-cadherin is a preliminary step in epithelial to mesenchymal transition; and (4) vimentin is a marker of mesenchymal phenotype. We hypothesized that stem cell subpopulations w  ...[more]

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