Ontology highlight
ABSTRACT:
SUBMITTER: Sun Y
PROVIDER: S-EPMC3401463 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Sun Yingjie Y Atas Evrim E Lindqvist Lisa L Sonenberg Nahum N Pelletier Jerry J Meller Amit A
Nucleic acids research 20120328 13
Eukaryotic translation initiation is a highly regulated process in protein synthesis. The principal translation initiation factor eIF4AI displays helicase activity, unwinding secondary structures in the mRNAs 5'-UTR. Single molecule fluorescence resonance energy transfer (sm-FRET) is applied here to directly observe and quantify the helicase activity of eIF4AI in the presence of the ancillary RNA-binding factor eIF4H. Results show that eIF4H can significantly enhance the helicase activity of eIF ...[more]