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Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells.

ABSTRACT: The advent of reprogramming and its impact on stem cell biology has renewed interest in lineage restriction in mammalian embryos, the source of embryonic (ES), epiblast (EpiSC), trophoblast (TS), and extraembryonic endoderm (XEN) stem cell lineages. Isolation of specific cell types during stem cell differentiation and reprogramming, and also directly from embryos, is a major technical challenge because few cell-surface proteins are known that can distinguish each cell type. We provide a large-scale proteomic resource of cell-surface proteins for the four embryo-derived stem cell lines. We validated 27 antibodies against lineage-specific cell-surface markers, which enabled investigation of specific cell populations during ES-EpiSC reprogramming and ES-to-XEN differentiation. Identified markers also allowed prospective isolation and characterization of viable lineage progenitors from blastocysts by flow cytometry. These results provide a comprehensive stem cell proteomic resource and enable new approaches to interrogate the mechanisms that regulate cell fate specification.

SUBMITTER: Rugg-Gunn PJ

PROVIDER: S-EPMC3405530 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells.

Rugg-Gunn Peter J PJ Cox Brian J BJ Lanner Fredrik F Sharma Parveen P Ignatchenko Vladimir V McDonald Angela C H AC Garner Jodi J Gramolini Anthony O AO Rossant Janet J Kislinger Thomas T

Developmental cell 20120315 4

The advent of reprogramming and its impact on stem cell biology has renewed interest in lineage restriction in mammalian embryos, the source of embryonic (ES), epiblast (EpiSC), trophoblast (TS), and extraembryonic endoderm (XEN) stem cell lineages. Isolation of specific cell types during stem cell differentiation and reprogramming, and also directly from embryos, is a major technical challenge because few cell-surface proteins are known that can distinguish each cell type. We provide a large-sc ...[more]

PMID: 22424930

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Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells.

Publications

Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells.

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