Unknown

Dataset Information

0

Capturing greater 16S rRNA gene sequence diversity within the domain Bacteria.


ABSTRACT: A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of taxonomic diversity with around a 3-fold increase in recovery of sequences from candidate divisions.

SUBMITTER: Winsley T 

PROVIDER: S-EPMC3406127 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Capturing greater 16S rRNA gene sequence diversity within the domain Bacteria.

Winsley T T   van Dorst J M JM   Brown M V MV   Ferrari B C BC  

Applied and environmental microbiology 20120608 16


A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of taxonomic diversity with around a 3-fold increase in recovery of sequences from candidate  ...[more]

Similar Datasets

| S-EPMC104915 | biostudies-literature
| S-EPMC154644 | biostudies-literature
| S-EPMC2893482 | biostudies-literature
| S-EPMC3812885 | biostudies-literature
| S-EPMC4726496 | biostudies-literature
| S-EPMC4913878 | biostudies-literature
| S-EPMC5856467 | biostudies-literature
| S-EPMC2732032 | biostudies-literature
| S-EPMC168236 | biostudies-other
| S-EPMC5675859 | biostudies-literature