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Capturing greater 16S rRNA gene sequence diversity within the domain Bacteria.

ABSTRACT: A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of taxonomic diversity with around a 3-fold increase in recovery of sequences from candidate divisions.

SUBMITTER: Winsley T 

PROVIDER: S-EPMC3406127 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Capturing greater 16S rRNA gene sequence diversity within the domain Bacteria.

Winsley T T   van Dorst J M JM   Brown M V MV   Ferrari B C BC  

Applied and environmental microbiology 20120608 16

A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of taxonomic diversity with around a 3-fold increase in recovery of sequences from candidate  ...[more]

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