Dataset Information

Galectin-1, a gene preferentially expressed at the tumor margin, promotes glioblastoma cell invasion.

ABSTRACT:

Background

High-grade gliomas, including glioblastomas (GBMs), are recalcitrant to local therapy in part because of their ability to invade the normal brain parenchyma surrounding these tumors. Animal models capable of recapitulating glioblastoma invasion may help identify mediators of this aggressive phenotype.

Methods

Patient-derived glioblastoma lines have been propagated in our laboratories and orthotopically xenografted into the brains of immunocompromized mice. Invasive cells at the tumor periphery were isolated using laser capture microdissection. The mRNA expression profile of these cells was compared to expression at the tumor core, using normal mouse brain to control for host contamination. Galectin-1, a target identified by screening the resulting data, was stably over-expressed in the U87MG cell line. Sub-clones were assayed for attachment, proliferation, migration, invasion, and in vivo tumor phenotype.

Results

Expression microarray data identified galectin-1 as the most potent marker (p-value 4.0 x 10-8) to identify GBM cells between tumor-brain interface as compared to the tumor core. Over-expression of galectin-1 enhanced migration and invasion in vitro. In vivo, tumors expressing high galectin-1 levels showed enhanced invasion and decreased host survival.

Conclusions

In conclusion, cells at the margin of glioblastoma, in comparison to tumor core cells, have enhanced expression of mediators of invasion. Galectin-1 is likely one such mediator. Previous studies, along with the current one, have proven galectin-1 to be important in the migration and invasion of glioblastoma cells, in GBM neoangiogenesis, and also, potentially, in GBM immune privilege. Targeting this molecule may offer clinical improvement to the current standard of glioblastoma therapy, i.e. radiation, temozolomide, anti-angiogenic therapy, and vaccinotherapy.

SUBMITTER: Toussaint LG

PROVIDER: S-EPMC3407025 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Publications

Galectin-1, a gene preferentially expressed at the tumor margin, promotes glioblastoma cell invasion.

Toussaint L Gerard LG Nilson Allan E AE Goble Jennie M JM Ballman Karla V KV James C David CD Lefranc Florence F Kiss Robert R Uhm Joon H JH

Molecular cancer 20120514

<h4>Background</h4>High-grade gliomas, including glioblastomas (GBMs), are recalcitrant to local therapy in part because of their ability to invade the normal brain parenchyma surrounding these tumors. Animal models capable of recapitulating glioblastoma invasion may help identify mediators of this aggressive phenotype.<h4>Methods</h4>Patient-derived glioblastoma lines have been propagated in our laboratories and orthotopically xenografted into the brains of immunocompromized mice. Invasive cell ...[more]

PMID: 22583806

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Dataset Information

Galectin-1, a gene preferentially expressed at the tumor margin, promotes glioblastoma cell invasion.

Background

Methods

Results

Conclusions

Publications

Galectin-1, a gene preferentially expressed at the tumor margin, promotes glioblastoma cell invasion.

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