A rapid survival assay to measure drug-induced cytotoxicity and cell cycle effects.

ABSTRACT: We describe a rapid method to accurately measure the cytotoxicity of mammalian cells upon exposure to various drugs. Using this assay, we obtain survival data in a fraction of the time required to perform the traditional clonogenic survival assay, considered the gold standard. The dynamic range of the assay allows sensitivity measurements on a multi-log scale allowing better resolution of comparative sensitivities. Moreover, the results obtained contain additional information on cell cycle effects of the drug treatment. Cell survival is obtained from a quantitative comparison of proliferation between drug-treated and untreated cells. During the assay, cells are treated with a drug and, following a recovery period, allowed to proliferate in the presence of bromodeoxyuridine (BrdU). Cells th

SUBMITTER: Valiathan C 

PROVIDER: S-EPMC3408220 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature