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Inactivation of a single gene enables microaerobic growth of the obligate anaerobe Bacteroides fragilis.


ABSTRACT: Bacteroides fragilis can replicate in atmospheres containing ?0.05% oxygen, but higher concentrations arrest growth by an unknown mechanism. Here we show that inactivation of a single gene, oxe (i.e., oxygen enabled) in B. fragilis allows for growth in concentrations as high as 2% oxygen while increasing the tolerance of this organism to room air. Known components of the oxidative stress response including the ahpC, kat, batA-E, and tpx genes were not individually important for microaerobic growth. However, a ?oxe strain scavenged H(2)O(2) at a faster rate than WT, indicating that reactive oxygen species may play a critical role in limiting growth of this organism to low-oxygen environments. Clinical isolates of B. fragilis displayed a greater capacity for growth under microaerobic conditions than fecal isolates, with some encoding polymorphisms in oxe. Additionally, isolation of oxygen-enabled mutants of Bacteroides thetaiotaomicron suggests that Oxe may mediate growth arrest of other anaerobes in oxygenated environments.

SUBMITTER: Meehan BM 

PROVIDER: S-EPMC3409759 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Inactivation of a single gene enables microaerobic growth of the obligate anaerobe Bacteroides fragilis.

Meehan Brian M BM   Baughn Anthony D AD   Gallegos Rene R   Malamy Michael H MH  

Proceedings of the National Academy of Sciences of the United States of America 20120709 30


Bacteroides fragilis can replicate in atmospheres containing ≤0.05% oxygen, but higher concentrations arrest growth by an unknown mechanism. Here we show that inactivation of a single gene, oxe (i.e., oxygen enabled) in B. fragilis allows for growth in concentrations as high as 2% oxygen while increasing the tolerance of this organism to room air. Known components of the oxidative stress response including the ahpC, kat, batA-E, and tpx genes were not individually important for microaerobic grow  ...[more]

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