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Insights into salt tolerance from the genome of Thellungiella salsuginea.

ABSTRACT: Thellungiella salsuginea, a close relative of Arabidopsis, represents an extremophile model for abiotic stress tolerance studies. We present the draft sequence of the T. salsuginea genome, assembled based on ~134-fold coverage to seven chromosomes with a coding capacity of at least 28,457 genes. This genome provides resources and evidence about the nature of defense mechanisms constituting the genetic basis underlying plant abiotic stress tolerance. Comparative genomics and experimental analyses identified genes related to cation transport, abscisic acid signaling, and wax production prominent in T. salsuginea as possible contributors to its success in stressful environments.

SUBMITTER: Wu HJ

PROVIDER: S-EPMC3409768 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Insights into salt tolerance from the genome of Thellungiella salsuginea.

Wu Hua-Jun HJ Zhang Zhonghui Z Wang Jun-Yi JY Oh Dong-Ha DH Dassanayake Maheshi M Liu Binghang B Huang Quanfei Q Sun Hai-Xi HX Xia Ran R Wu Yaorong Y Wang Yi-Nan YN Yang Zhao Z Liu Yang Y Zhang Wanke W Zhang Huawei H Chu Jinfang J Yan Cunyu C Fang Shuang S Zhang Jinsong J Wang Yiqin Y Zhang Fengxia F Wang Guodong G Lee Sang Yeol SY Cheeseman John M JM Yang Bicheng B Li Bo B Min Jiumeng J Yang Linfeng L Wang Jun J Chu Chengcai C Chen Shou-Yi SY Bohnert Hans J HJ Zhu Jian-Kang JK Wang Xiu-Jie XJ Xie Qi Q

Proceedings of the National Academy of Sciences of the United States of America 20120709 30

Thellungiella salsuginea, a close relative of Arabidopsis, represents an extremophile model for abiotic stress tolerance studies. We present the draft sequence of the T. salsuginea genome, assembled based on ~134-fold coverage to seven chromosomes with a coding capacity of at least 28,457 genes. This genome provides resources and evidence about the nature of defense mechanisms constituting the genetic basis underlying plant abiotic stress tolerance. Comparative genomics and experimental analyses ...[more]

PMID: 22778405

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Project description:Containing both AP2 domain and B3 domain, RAV (Related to ABI3/VP1) transcription factors are involved in diverse functions in higher plants. A total of eight TsRAV genes were isolated from the genome of Thellungiella salsuginea and could be divided into two groups (A- and B-group) based on their sequence similarity. The mRNA abundance of all Thellungiella salsuginea TsRAVs followed a gradual decline during seed germination. In Thellungiella salsuginea seedling, transcripts of TsRAVs in the group A (A-TsRAVs) were gradually and moderately reduced by salt treatment but rapidly and severely repressed by ABA treatment. In comparison, with a barely detectable constitutive expression, the transcriptional level of TsRAVs in the group B (B-TsRAVs) exhibited a moderate induction in cotyledons when confronted with ABA. We then produced the "gain-of-function" transgenic Arabidopsis plants for each TsRAV gene and found that only 35S:A-TsRAVs showed weak growth retardation including reduced root elongation, suggesting their roles in negatively controlling plant growth. Under normal conditions, the germination process of all TsRAVs overexpressing transgenic seeds was inhibited with a stronger effect observed in 35S:A-TsRAVs seeds than in 35S:B-TsRAVs seeds. With the presence of NaCl, seed germination and seedling root elongation of all plants including wild type and 35S:TsRAVs plants were retarded and a more severe inhibition occurred to the 35S:A-TsRAV transgenic plants. ABA treatment only negatively affected the germination rates of 35S:A-TsRAV transgenic seeds but not those of 35S:B-TsRAV transgenic seeds. All 35S:TsRAVs transgenic plants showed a similar degree of reduction in root growth compared with untreated seedlings in the presence of ABA. Furthermore, the cotyledon greening/expansion was more severely inhibited 35S:A-TsRAVs than in 35S:B-TsRAVs seedlings. Upon water deficiency, with a wider opening of stomata, 35S:A-TsRAVs plants experienced a faster transpirational water loss than wild type and 35S:B-TsRAVs lines. Taken together, our results suggest that two groups of TsRAVs perform distinct regulating roles during plant growth and abiotic defense including drought and salt, and A-TsRAVs are more likely than B-TsRAVs to act as negative regulators in the above-mentioned biological processes.

Dataset Information

Insights into salt tolerance from the genome of Thellungiella salsuginea.

Publications

Insights into salt tolerance from the genome of Thellungiella salsuginea.

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