Ontology highlight
ABSTRACT:
SUBMITTER: Wang P
PROVIDER: S-EPMC3411363 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Wang Pei P McKnight Kristen D KD Wong David J DJ Rodriguez Ryan T RT Sugiyama Takuya T Gu Xueying X Ghodasara Amar A Qu Kun K Chang Howard Y HY Kim Seung K SK
Stem cells and development 20120229 12
Embryonic definitive endoderm (DE) generates the epithelial compartment of vital organs such as liver, pancreas, and intestine. However, purification of DE in mammals has not been achieved, limiting the molecular "definition" of endoderm, and hindering our understanding of DE development and attempts to produce endoderm from sources such as embryonic stem (ES) cells. Here, we describe purification of mouse DE using fluorescence-activated cell sorting (FACS) and mice harboring a transgene encodin ...[more]