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Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells.


ABSTRACT: Hydrogen sulfide (H(2)S) can be endogenously generated from cystathionine gamma-lyase (CSE) in cardiovascular system, offering a cardiovascular protection. It is also known that the lower risk of cardiovascular diseases in female is partially attributed to the protective effect of estrogen. The current study explores the interaction of H(2)S and estrogen on smooth muscle cell (SMC) growth. In the present study, we found that the proliferation of cultured vascular SMCs isolated from wild-type mice (WT-SMCs) was inhibited, but that from CSE gene knockout mice (CSE-KO-SMCs) increased, by estrogen treatments. The expression of estrogen receptor ? (ER?), but not ER?, was significantly decreased in CSE-KO-SMCs compared with that in WT-SMCs. Exogenously applied H(2)S markedly increased ER? but not ER? expression. In addition, the inhibition of ER activation and knockdown of ER? expression in WT-SMCs or the overexpression of ER? in CSE-KO-SMCs reversed the respective effects of estrogen on cell proliferation. The expression of cyclin D1 was reduced in WT-SMCs but increased in CSE-KO-SMCs after estrogen treatments, which was reversed by knockdown of ER? in WT-SMCs or overexpression of ER? in CSE-KO-SMCs, respectively. The overexpression of cyclin D1 in WT-SMCs or knockdown of cyclin D1 expression in CSE-KO-SMCs reversed the effects of estrogen on cell proliferation. These results suggest that H(2)S mediates estrogen-inhibited proliferation of SMCs via selective activation of ER?/cyclin D1 pathways.

SUBMITTER: Li H 

PROVIDER: S-EPMC3411693 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Interaction of hydrogen sulfide and estrogen on the proliferation of vascular smooth muscle cells.

Li Hongzhu H   Mani Sarathi S   Cao Wei W   Yang Guangdong G   Lai Christopher C   Wu Lingyun L   Wang Rui R  

PloS one 20120803 8


Hydrogen sulfide (H(2)S) can be endogenously generated from cystathionine gamma-lyase (CSE) in cardiovascular system, offering a cardiovascular protection. It is also known that the lower risk of cardiovascular diseases in female is partially attributed to the protective effect of estrogen. The current study explores the interaction of H(2)S and estrogen on smooth muscle cell (SMC) growth. In the present study, we found that the proliferation of cultured vascular SMCs isolated from wild-type mic  ...[more]

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