Unknown

Dataset Information

0

MiR-29ab1 deficiency identifies a negative feedback loop controlling Th1 bias that is dysregulated in multiple sclerosis.


ABSTRACT: Th cell programming and function is tightly regulated by complex biological networks to prevent excessive inflammatory responses and autoimmune disease. The importance of microRNAs (miRNAs) in this process is highlighted by the preferential Th1 polarization of Dicer-deficient T cells that lack miRNAs. Using genetic knockouts, we demonstrate that loss of endogenous miR-29, derived from the miR-29ab1 genomic cluster, results in unrestrained T-bet expression and IFN-? production. miR-29b regulates T-bet and IFN-? via a direct interaction with the 3' untranslated regions, and IFN-? itself enhances miR-29b expression, establishing a novel regulatory feedback loop. miR-29b is increased in memory CD4(+) T cells from multiple sclerosis (MS) patients, which may reflect chronic Th1 inflammation. However, miR-29b levels decrease significantly upon T cell activation in MS patients, suggesting that this feedback loop is dysregulated in MS patients and may contribute to chronic inflammation. miR-29 thus serves as a novel regulator of Th1 differentiation, adding to the understanding of T cell-intrinsic regulatory mechanisms that maintain a balance between protective immunity and autoimmunity.

SUBMITTER: Smith KM 

PROVIDER: S-EPMC3411895 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

miR-29ab1 deficiency identifies a negative feedback loop controlling Th1 bias that is dysregulated in multiple sclerosis.

Smith Kristen M KM   Guerau-de-Arellano Mireia M   Costinean Stefan S   Williams Jessica L JL   Bottoni Arianna A   Mavrikis Cox Gina G   Satoskar Abhay R AR   Croce Carlo M CM   Racke Michael K MK   Lovett-Racke Amy E AE   Whitacre Caroline C CC  

Journal of immunology (Baltimore, Md. : 1950) 20120706 4


Th cell programming and function is tightly regulated by complex biological networks to prevent excessive inflammatory responses and autoimmune disease. The importance of microRNAs (miRNAs) in this process is highlighted by the preferential Th1 polarization of Dicer-deficient T cells that lack miRNAs. Using genetic knockouts, we demonstrate that loss of endogenous miR-29, derived from the miR-29ab1 genomic cluster, results in unrestrained T-bet expression and IFN-γ production. miR-29b regulates  ...[more]

Similar Datasets

| S-EPMC6190839 | biostudies-other
| S-EPMC2757731 | biostudies-literature
| S-EPMC8195427 | biostudies-literature
| S-EPMC3514527 | biostudies-other
| S-EPMC5299403 | biostudies-literature
| S-EPMC3025456 | biostudies-literature
| S-EPMC4614452 | biostudies-literature
| S-EPMC4521751 | biostudies-literature
| S-EPMC4151748 | biostudies-literature
| S-EPMC5529933 | biostudies-other