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Regulation of multiple cytokine signalling pathways by SOCS3 is independent of SOCS2.

ABSTRACT: Suppressor of cytokine signalling (SOCS) 3 is an essential regulator of cytokine signalling, and in turn its expression is tightly regulated. Data from overexpression studies in cell lines suggest that SOCS2 regulates SOCS3 protein degradation, by forming a molecular bridge to an E3 ubiquitin-ligase complex. Whether this regulation is relevant in primary cells is unknown. In this study, we utilized Socs2( - / - ) mice to examine the role of SOCS2 in modulating SOCS3 expression and degradation, and its impact on interleukin-2 (IL-2) and IL-6 signalling in primary haemopoietic cells. Both biochemical and biological analyses demonstrated unperturbed SOCS3 expression and cytokine signalling in the absence of SOCS2. Our results suggest that SOCS2 is not a physiological regulator of SOCS3 expression and action in primary haemopoietic cells.

SUBMITTER: Kiu H 

PROVIDER: S-EPMC3412171 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature

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Regulation of multiple cytokine signalling pathways by SOCS3 is independent of SOCS2.

Kiu Hiu H   Greenhalgh Christopher J CJ   Thaus Anne A   Hilton Douglas J DJ   Nicola Nicos A NA   Alexander Warren S WS   Roberts Andrew W AW  

Growth factors (Chur, Switzerland) 20091201 6

Suppressor of cytokine signalling (SOCS) 3 is an essential regulator of cytokine signalling, and in turn its expression is tightly regulated. Data from overexpression studies in cell lines suggest that SOCS2 regulates SOCS3 protein degradation, by forming a molecular bridge to an E3 ubiquitin-ligase complex. Whether this regulation is relevant in primary cells is unknown. In this study, we utilized Socs2( - / - ) mice to examine the role of SOCS2 in modulating SOCS3 expression and degradation, a  ...[more]