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Crystallization and preliminary X-ray diffraction analysis of a novel type of phosphoserine phosphatase from Hydrogenobacter thermophilus TK-6.


ABSTRACT: Two novel-type phosphoserine phosphatases (PSPs) with unique substrate specificity from the thermophilic and hydrogen-oxidizing bacterium Hydrogenobacter thermophilus TK-6 have previously been identified. Here, one of the PSPs (iPSP1) was heterologously expressed in Escherichia coli, purified and crystallized. Diffraction-quality crystals were obtained by the sitting-drop vapour-diffusion method using PEG 4000 as the precipitant. Two diffraction data sets with resolution ranges of 45.0-2.50 and 45.0-1.50?Å were collected from a single crystal and were merged to give a highly complete data set. The space group of the crystal was identified as primitive orthorhombic P2(1)2(1)2(1), with unit-cell parameters a = 49.8, b = 73.6, c = 124.3?Å. The calculated Matthews coefficient (V(M) = 2.32?Å(3)?Da(-1)) indicated that the crystal contained one iPSP1 complex per asymmetric unit.

SUBMITTER: Chiba Y 

PROVIDER: S-EPMC3412771 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray diffraction analysis of a novel type of phosphoserine phosphatase from Hydrogenobacter thermophilus TK-6.

Chiba Yoko Y   Horita Shoichiro S   Ohtsuka Jun J   Arai Hiroyuki H   Nagata Koji K   Igarashi Yasuo Y   Tanokura Masaru M   Ishii Masaharu M  

Acta crystallographica. Section F, Structural biology and crystallization communications 20120731 Pt 8


Two novel-type phosphoserine phosphatases (PSPs) with unique substrate specificity from the thermophilic and hydrogen-oxidizing bacterium Hydrogenobacter thermophilus TK-6 have previously been identified. Here, one of the PSPs (iPSP1) was heterologously expressed in Escherichia coli, purified and crystallized. Diffraction-quality crystals were obtained by the sitting-drop vapour-diffusion method using PEG 4000 as the precipitant. Two diffraction data sets with resolution ranges of 45.0-2.50 and  ...[more]

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