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Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses.

ABSTRACT: A multifunctional liquid chromatography system that performs 1-dimensional, 2-dimensional (strong cation exchange/reverse phase liquid chromatography or SCX/RPLC) separations and online phosphopeptide enrichment using a single binary nanoflow pump has been developed. With a simple operation of a function selection valve equipped with a SCX column and a TiO2 (titanium dioxide) column, a fully automated selection of three different experiment modes was achieved. Because the current system uses essentially the same solvent flow paths, the same trap column, and the same separation column for reverse-phase separation of 1D, 2D, and online phosphopeptides enrichment experiments, the elution time information obtained from these experiments is in excellent agreement, which facilitates correlating peptide information from different experiments. The final reverse-phase separation of the three experiments is completely decoupled from all of the function selection processes; thereby salts or acids from SCX or TiO2 column do not affect the efficiency of the reverse-phase separation.

SUBMITTER: Lee JH

PROVIDER: S-EPMC3412908 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses.

Lee Jung Hwa JH Hyung Seok-Won SW Mun Dong-Gi DG Jung Hee-Jung HJ Kim Hokeun H Lee Hangyeore H Kim Su-Jin SJ Park Kyong Soo KS Moore Ronald J RJ Smith Richard D RD Lee Sang-Won SW

Journal of proteome research 20120705 8

A multifunctional liquid chromatography system that performs 1-dimensional, 2-dimensional (strong cation exchange/reverse phase liquid chromatography or SCX/RPLC) separations and online phosphopeptide enrichment using a single binary nanoflow pump has been developed. With a simple operation of a function selection valve equipped with a SCX column and a TiO2 (titanium dioxide) column, a fully automated selection of three different experiment modes was achieved. Because the current system uses ess ...[more]

PMID: 22709424

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Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses.

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Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses.

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