Ontology highlight
ABSTRACT:
SUBMITTER: Sheng Y
PROVIDER: S-EPMC3412965 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Sheng Yi Y Hong Jenny H JH Doherty Ryan R Srikumar Tharan T Shloush Jonathan J Avvakumov George V GV Walker John R JR Xue Sheng S Neculai Dante D Wan Janet W JW Kim Sung K SK Arrowsmith Cheryl H CH Raught Brian B Dhe-Paganon Sirano S
Molecular & cellular proteomics : MCP 20120410 8
Here we describe a systematic structure-function analysis of the human ubiquitin (Ub) E2 conjugating proteins, consisting of the determination of 15 new high-resolution three-dimensional structures of E2 catalytic domains, and autoubiquitylation assays for 26 Ub-loading E2s screened against a panel of nine different HECT (homologous to E6-AP carboxyl terminus) E3 ligase domains. Integration of our structural and biochemical data revealed several E2 surface properties associated with Ub chain bui ...[more]