Ontology highlight
ABSTRACT:
SUBMITTER: Tomkuviene M
PROVIDER: S-EPMC3413156 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Tomkuviene Migle M Clouet-d'Orval Béatrice B Cerniauskas Ignas I Weinhold Elmar E Klimasauskas Saulius S
Nucleic acids research 20120507 14
Biophysical and mechanistic investigation of RNA function requires site-specific incorporation of spectroscopic and chemical probes, which is difficult to achieve using current technologies. We have in vitro reconstituted a functional box C/D small ribonucleoprotein RNA 2'-O-methyltransferase (C/D RNP) from the thermophilic archaeon Pyrococcus abyssi and demonstrated its ability to transfer a prop-2-ynyl group from a synthetic cofactor analog to a series of preselected target sites in model tRNA ...[more]