Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate.
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ABSTRACT:
SUBMITTER: Lai RY
PROVIDER: S-EPMC3415583 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Publications
Thiamin pyrimidine biosynthesis in Candida albicans : a remarkable reaction between histidine and pyridoxal phosphate.
Lai Rung-Yi RY Huang Siyu S Fenwick Michael K MK Hazra Amrita A Zhang Yang Y Rajashankar Kanagalaghatta K Philmus Benjamin B Kinsland Cynthia C Sanders Jennie Mansell JM Ealick Steven E SE Begley Tadhg P TP
Journal of the American Chemical Society 20120524 22
In Saccharomyces cerevisiae , thiamin pyrimidine is formed from histidine and pyridoxal phosphate (PLP). The origin of all of the pyrimidine atoms has been previously determined using labeling studies and suggests that the pyrimidine is formed using remarkable chemistry that is without chemical or biochemical precedent. Here we report the overexpression of the closely related Candida albicans pyrimidine synthase (THI5p) and the reconstitution and preliminary characterization of the enzymatic act ...[more]
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