SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing.
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ABSTRACT:
SUBMITTER: Lindner H
PROVIDER: S-EPMC3416015 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Publications
SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing.
Lindner Heike H Raissig Michael T MT Sailer Christian C Shimosato-Asano Hiroko H Bruggmann Rémy R Grossniklaus Ueli U
Genetics 20120529 4
We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds. ...[more]
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