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Identification and molecular characterization of class 1 integrons in multiresistant Escherichia coli isolates from poultry litter.

ABSTRACT: This study describes the prevalence of arrays of class 1 integron cassettes and Qnr determinants (A, B, and S) in 19 fluoroquinolone-resistant Escherichia coli isolates from chicken litter. qnrS and qnrA were the predominant genes in these fluoroquinolone-resistant isolates, and an uncommon array of aacA4-catB3-dfrA1 gene cassettes from a class1 integron was found. Additionally, aadA1 and dfrA1 gene cassettes, encoding resistance to streptomycin and trimethoprim, constituted the most common genes identified and was located on megaplasmids as well on the chromosome. Antibiotic resistance, pulsed-field gel electrophoresis (PFGE), and plasmid data suggest a genetically diverse origin of poultry E. coli isolates.

SUBMITTER: Ponce-Rivas E

PROVIDER: S-EPMC3416406 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Identification and molecular characterization of class 1 integrons in multiresistant Escherichia coli isolates from poultry litter.

Ponce-Rivas Elizabeth E Muñoz-Márquez María-Enriqueta ME Khan Ashraf A AA

Applied and environmental microbiology 20120525 15

This study describes the prevalence of arrays of class 1 integron cassettes and Qnr determinants (A, B, and S) in 19 fluoroquinolone-resistant Escherichia coli isolates from chicken litter. qnrS and qnrA were the predominant genes in these fluoroquinolone-resistant isolates, and an uncommon array of aacA4-catB3-dfrA1 gene cassettes from a class1 integron was found. Additionally, aadA1 and dfrA1 gene cassettes, encoding resistance to streptomycin and trimethoprim, constituted the most common gene ...[more]

PMID: 22635994

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