One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies.
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ABSTRACT:
SUBMITTER: Jeong JY
PROVIDER: S-EPMC3416421 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Publications
One-step sequence- and ligation-independent cloning as a rapid and versatile cloning method for functional genomics studies.
Jeong Jae-Yeon JY Yim Hyung-Soon HS Ryu Ji-Young JY Lee Hyun Sook HS Lee Jung-Hyun JH Seen Dong-Seung DS Kang Sung Gyun SG
Applied and environmental microbiology 20120518 15
We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature. ...[more]
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