Differential in vivo gene expression of major Leptospira proteins in resistant or susceptible animal models.
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ABSTRACT:
SUBMITTER: Matsui M
PROVIDER: S-EPMC3416592 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
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Differential in vivo gene expression of major Leptospira proteins in resistant or susceptible animal models.
Matsui Mariko M Soupé Marie-Estelle ME Becam Jérôme J Goarant Cyrille C
Applied and environmental microbiology 20120622 17
Transcripts of Leptospira 16S rRNA, FlaB, LigB, LipL21, LipL32, LipL36, LipL41, and OmpL37 were quantified in the blood of susceptible (hamsters) and resistant (mice) animal models of leptospirosis. We first validated adequate reference genes and then evaluated expression patterns in vivo compared to in vitro cultures. LipL32 expression was downregulated in vivo and differentially regulated in resistant and susceptible animals. FlaB expression was also repressed in mice but not in hamsters. In c ...[more]
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