Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
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ABSTRACT:
SUBMITTER: Morlan JD
PROVIDER: S-EPMC3416766 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
Morlan John D JD Qu Kunbin K Sinicropi Dominick V DV
PloS one 20120810 8
We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Nex ...[more]
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