Templated chemistry for monitoring damage and repair directly in duplex DNA.
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ABSTRACT:
SUBMITTER: Lee SH
PROVIDER: S-EPMC3417126 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Publications
Templated chemistry for monitoring damage and repair directly in duplex DNA.
Lee Seoung Ho SH Wang Shenliang S Kool Eric T ET
Chemical communications (Cambridge, England) 20120710 65
We report the fluorogenic detection of the product of base excision repair (an abasic site) in a specific sequence of duplex DNA. This is achieved by DNA-templated chemistry, employing triple helix-forming probes that contain unnatural nucleobases designed to selectively recognize the site of a missing base. Light-up signals of up to 36-fold were documented, and probes could be used to monitor enzymatic removal of a damaged base. ...[more]
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