ADAMTS13 reduces VWF-mediated acute inflammation following focal cerebral ischemia in mice.

ABSTRACT:

Background

ADAMTS13 cleaves hyperactive ultra-large von Willebrand factor (ULVWF) multimers into smaller and less active forms. It remains unknown whether VWF-mediated inflammatory processes play a role in the enhanced brain injury due to ADAMTS13 deficiency.

Objective

We tested the hypothesis that the deleterious effect of ADAMTS13 deficiency on ischemic brain injury is mediated through VWF-dependent enhanced vascular inflammation.

Methods

Transient focal cerebral ischemia was induced by 60 min of occlusion of the right middle cerebral artery. Myeloperoxidase (MPO) activity and inflammatory cytokines in the infarcted region were evaluated 23 h after reperfusion injury. Neutrophil infiltration within the infarct and surrounding areas was quantitated by immunohistochem

SUBMITTER: Khan MM 

PROVIDER: S-EPMC3419774 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature