Accurate and rapid novel genetic diagnosis for detection of sentinel lymph node metastasis in breast cancer patients.

ABSTRACT:

Background

The transcription-reverse transcription concerted reaction (TRC) test is a novel molecular-based procedure, which can assess nodal metastasis accurately and quickly. We examined the usefulness of the TRC test with a double marker, cytokeratin 19 (CK19) and carcinoembryonic antigen (CEA) mRNA, to detect sentinel lymph nodes (SLN) metastasis in breast cancer patients.

Methods

A total of 264 SLNs from 131 breast cancer patients were assigned to a training set (109 SLNs from 50 patients) and validation set (155 SLNs from 81 patients). Cytokeratin 19 and CEA mRNA were detected by TRC tests, and the sensitivity and specificity of the SLN metastasis between the TRC and histology cohorts were compared.

Results

Mean copy numbers of CK19 and CEA by TRC tests were inc

SUBMITTER: Iinuma H 

PROVIDER: S-EPMC3419953 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature