Ontology highlight
ABSTRACT:
SUBMITTER: Nejepinska J
PROVIDER: S-EPMC3420890 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
PloS one 20120816 8
Transient plasmid transfection is a common approach in studies in cultured mammalian cells. To examine behavior of transfected plasmids, we analyzed their transcriptional landscape by deep sequencing. We have found that the entire plasmid sequence is transcribed at different levels. Spurious transcription may have undesirable effects as some plasmids, when co-transfected, inhibited expression of luciferase reporters in a dose-dependent manner. In one case, we attributed this effect to a Kan/Neo ...[more]