Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups.
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ABSTRACT:
SUBMITTER: Doumith M
PROVIDER: S-EPMC3421818 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Publications
Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups.
Doumith M M Day M J MJ Hope R R Wain J J Woodford N N
Journal of clinical microbiology 20120711 9
Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data. ...[more]
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