Ontology highlight
ABSTRACT:
SUBMITTER: Groll M
PROVIDER: S-EPMC34229 | biostudies-literature | 1999 Sep
REPOSITORIES: biostudies-literature
Groll M M Heinemeyer W W Jäger S S Ullrich T T Bochtler M M Wolf D H DH Huber R R
Proceedings of the National Academy of Sciences of the United States of America 19990901 20
We present a biochemical and crystallographic characterization of active site mutants of the yeast 20S proteasome with the aim to characterize substrate cleavage specificity, subunit intermediate processing, and maturation. beta1(Pre3), beta2(Pup1), and beta5(Pre2) are responsible for the postacidic, tryptic, and chymotryptic activity, respectively. The maturation of active subunits is independent of the presence of other active subunits and occurs by intrasubunit autolysis. The propeptides of b ...[more]