Ontology highlight
ABSTRACT:
SUBMITTER: Kuznetsov NA
PROVIDER: S-EPMC3424566 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Kuznetsov Nikita A NA Vorobjev Yuri N YN Krasnoperov Lev N LN Fedorova Olga S OS
Nucleic acids research 20120514 15
Formamidopyrimidine-DNA glycosylase, Fpg protein from Escherichia coli, initiates base excision repair in DNA by removing a wide variety of oxidized lesions. In this study, we perform thermodynamic analysis of the multi-stage interaction of Fpg with specific DNA-substrates containing 7,8-dihydro-8-oxoguanosine (oxoG), or tetrahydrofuran (THF, an uncleavable abasic site analog) and non-specific (G) DNA-ligand based on stopped-flow kinetic data. Pyrrolocytosine, highly fluorescent analog of the na ...[more]