3D multi-isotope imaging mass spectrometry reveals penetration of 18O-trehalose in mouse sperm nucleus.
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ABSTRACT:
SUBMITTER: Lechene CP
PROVIDER: S-EPMC3428319 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
3D multi-isotope imaging mass spectrometry reveals penetration of 18O-trehalose in mouse sperm nucleus.
Lechene Claude P CP Lee Gloria Y GY Poczatek J Collin JC Toner Mehmet M Biggers John D JD
PloS one 20120827 8
The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permittin ...[more]
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