Ontology highlight
ABSTRACT:
SUBMITTER: Auld DS
PROVIDER: S-EPMC3430137 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Journal of medicinal chemistry 20090301 5
We measured the "druggability" of the ATP-dependent luciferase derived from the firefly Photuris pennsylvanica that was optimized using directed evolution (Ultra-Glo, Promega). Quantitative high-throughput screening (qHTS) was used to determine IC(50)s of 198899 samples against a formulation of Ultra-Glo luciferase (Kinase-Glo). We found that only 0.1% of the Kinase-Glo inhibitors showed an IC(50) < 10 microM compared to 0.9% found from a previous qHTS against the firefly luciferase from Photinu ...[more]