Ontology highlight
ABSTRACT:
SUBMITTER: Phanstiel DH
PROVIDER: S-EPMC3432645 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Phanstiel Douglas H DH Brumbaugh Justin J Wenger Craig D CD Tian Shulan S Probasco Mitchell D MD Bailey Derek J DJ Swaney Danielle L DL Tervo Mark A MA Bolin Jennifer M JM Ruotti Victor V Stewart Ron R Thomson James A JA Coon Joshua J JJ
Nature methods 20110911 10
Combining high-mass-accuracy mass spectrometry, isobaric tagging and software for multiplexed, large-scale protein quantification, we report deep proteomic coverage of four human embryonic stem cell and four induced pluripotent stem cell lines in biological triplicate. This 24-sample comparison resulted in a very large set of identified proteins and phosphorylation sites in pluripotent cells. The statistical analysis afforded by our approach revealed subtle but reproducible differences in protei ...[more]