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Bioorthogonal turn-on probes for imaging small molecules inside living cells.

ABSTRACT: Glowing tags: a series of activatable ("turn-on") tetrazine-conjugated fluorescent probes was developed, which react rapidly in an inverse-electron-demand [4+2] cycloaddition with strained dienophiles such as trans-cyclooctene, thereby strongly increasing the fluorescence intensity. The novel turn-on probes were applied for intracellular live-cell imaging of a microtubuli-binding trans-cyclooctene modified taxol.

SUBMITTER: Devaraj NK 

PROVIDER: S-EPMC3433403 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Bioorthogonal turn-on probes for imaging small molecules inside living cells.

Devaraj Neal K NK   Hilderbrand Scott S   Upadhyay Rabi R   Mazitschek Ralph R   Weissleder Ralph R  

Angewandte Chemie (International ed. in English) 20100319 16

Glowing tags: a series of activatable ("turn-on") tetrazine-conjugated fluorescent probes was developed, which react rapidly in an inverse-electron-demand [4+2] cycloaddition with strained dienophiles such as trans-cyclooctene, thereby strongly increasing the fluorescence intensity. The novel turn-on probes were applied for intracellular live-cell imaging of a microtubuli-binding trans-cyclooctene modified taxol. ...[more]

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