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Direct observation of mammalian cell growth and size regulation.

ABSTRACT: We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.

SUBMITTER: Son S

PROVIDER: S-EPMC3433595 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Direct observation of mammalian cell growth and size regulation.

Son Sungmin S Tzur Amit A Weng Yaochung Y Jorgensen Paul P Kim Jisoo J Kirschner Marc W MW Manalis Scott R SR

Nature methods 20120805 9

We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis. ...[more]

PMID: 22863882

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