Ontology highlight
ABSTRACT:
SUBMITTER: Baruah K
PROVIDER: S-EPMC3437440 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Journal of molecular biology 20120405 1-2
Serum IgG is a potent inhibitor of monoclonal antibody (mAb) binding to the cell-surface Fcγ receptors (FcγRs), which mediate cytotoxic and phagocytic effector functions. Here, we show that this competition can be eliminated, selectively, by the introduction to serum of (i) an enzyme that displaces Fc from FcγRs and (ii) a modification present in the therapeutic mAb that renders it resistant to that enzyme. Specifically, we show that (i) EndoS (endoglycosidase S) cleaves only complex-type glycan ...[more]