First analysis of the secretome of the canine heartworm, Dirofilaria immitis.

ABSTRACT:

Background

The characterization of proteins released from filariae is an important step in addressing many of the needs in the diagnosis and treatment of these clinically important parasites, as well as contributing to a clearer understanding of their biology. This report describes findings on the proteins released during in vitro cultivation of adult Dirofilaria immitis , the causative agent of canine and feline heartworm disease. Differences in protein secretion among nematodes in vivo may relate to the ecological niche of each parasite and the pathological changes that they induce.

Methods

The proteins in the secretions of cultured adult worms were run on Tris-Glycine gels, bands separated and peptides from each band analysed by ultra mass spectrometry and compared with a

SUBMITTER: Geary J 

PROVIDER: S-EPMC3439246 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature