Genomics of adaptation during experimental evolution of the opportunistic pathogen Pseudomonas aeruginosa.

ABSTRACT: Adaptation is likely to be an important determinant of the success of many pathogens, for example when colonizing a new host species, when challenged by antibiotic treatment, or in governing the establishment and progress of long-term chronic infection. Yet, the genomic basis of adaptation is poorly understood in general, and for pathogens in particular. We investigated the genetics of adaptation to cystic fibrosis-like culture conditions in the presence and absence of fluoroquinolone antibiotics using the opportunistic pathogen Pseudomonas aeruginosa. Whole-genome sequencing of experimentally evolved isolates revealed parallel evolution at a handful of known antibiotic resistance genes. While the level of antibiotic resistance was largely determined by these known resistance genes, the co

SUBMITTER: Wong A 

PROVIDER: S-EPMC3441735 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature