Enzymatic synthesis and properties of uridine-5'-O-(2-thiodiphospho)-N-acetylglucosamine.
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ABSTRACT:
SUBMITTER: Cai L
PROVIDER: S-EPMC3441826 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
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Enzymatic synthesis and properties of uridine-5'-O-(2-thiodiphospho)-N-acetylglucosamine.
Cai Li L Ban Lan L Guan Wanyi W Mrksich Milan M Wang Peng George PG
Carbohydrate research 20110512 12
This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction. ...[more]
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