Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.
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ABSTRACT:
SUBMITTER: Milles S
PROVIDER: S-EPMC3441834 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
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Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range.
Milles Sigrid S Koehler Christine C Gambin Yann Y Deniz Ashok A AA Lemke Edward A EA
Molecular bioSystems 20121001 10
Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins. ...[more]
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