Ontology highlight
ABSTRACT:
SUBMITTER: Hillyer P
PROVIDER: S-EPMC3442264 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Hillyer Philippa P Mane Viraj P VP Schramm Lynnsie M LM Puig Montserrat M Verthelyi Daniela D Chen Aaron A Zhao Zeng Z Navarro Maria B MB Kirschman Kevin D KD Bykadi Srikant S Jubin Ronald G RG Rabin Ronald L RL
Immunology and cell biology 20120117 8
Recent genome-wide association studies suggest distinct roles for 12 human interferon-alpha (IFN-α) and 3 IFN-λ subtypes that may be elucidated by defining the expression patterns of these sets of genes. To overcome the impediment of high homology among each of the sets, we designed a quantitative real-time PCR assay that incorporates the use of molecular beacon and locked nucleic acid (LNA) probes, and in some instances, LNA oligonucleotide inhibitors. We then measured IFN subtype expression by ...[more]