Ontology highlight
ABSTRACT:
SUBMITTER: Klauschen F
PROVIDER: S-EPMC3443679 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Klauschen Frederick F Ishii Masaru M Qi Hai H Bajénoff Marc M Egen Jackson G JG Germain Ronald N RN Meier-Schellersheim Martin M
Nature protocols 20090820 9
The wealth of information available from advanced fluorescence imaging techniques used to analyze biological processes with high spatial and temporal resolution calls for high-throughput image analysis methods. Here, we describe a fully automated approach to analyzing cellular interaction behavior in 3D fluorescence microscopy images. As example application, we present the analysis of drug-induced and S1P(1)-knockout-related changes in bone-osteoclast interactions. Moreover, we apply our approac ...[more]